Coding

Part:BBa_K3468045

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase T72R

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to R at 72 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds stabilized α2-β3loop and α3-β5loop The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bond with 51-THR. after mutation to ARG, it formed hydrogen bond with 73-ASN and 148-LYS, which stabilized α2-β3loop and α3-β5loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 T72R in PyMOL
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Categories
Parameters
None